| It's a straightforward assignment, 47.|
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Alligators can be found in the swamp water surrounding the mansion in the mission Till Death Do Us Part in Hitman: Blood Money. They will eat any NPC's thrown into the water. Near the start of the mission, several wedding guests can be seen leisurely shooting alligators, an activity which Agent 47 can join in on. The target John LeBlanc also enjoys feeding alligators chicken.
Additionally, they are referenced in the mission Death on the Mississippi, where the targets the Gator Gang is named for them, and the other target Skip Muldoon is given a FN-2000 by some business contacts for alligator hunting. Strangely, alligators leave 47 alone, no matter how far into the swamp water he goes.